Composite

Part:BBa_K1136003:Design

Designed by: Simin Jiang   Group: iGEM13_Nevada   (2013-09-16)


An L-Arabinose controlled endolysin from bacteriophage XP15


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 65
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 328
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The endolysin gene was synthesized by GenScript to optimize codons and avoid illegal sites. The promotor and terminator were chosen for controllable expression, because the endolysin may be toxic in high copy number.


Source

The endolysin gene is from bacteriophage XP15. An E. coli promoter and terminator were used.

References